FEBS Letters | |
Insulin modulation of newly synthesized apolipoproteins B‐100 and B‐48 in human fetal intestine: Gene expression and mRNA editing are not involved | |
Sinnett, Daniel1  Nguyen, Thanh Dung1  Delvin, Edgard1  Ménard, Daniel2  Thibault, Louise1  Levy, Emile1  | |
[1] Departments of Nutrition, Pediatrics and Biochemistry, Centre de Recherche, Hôpital Sainte-Justine, Université de Montréal, 3175 Côte Ste-Catherine Road, Montreal, Québec H3T 1C5, Canada;Groupe de Recherche en Biologie de Développement, Département d'Anatomie et de Biologie Cellulaire, Faculté de Médecine, Université de Sherbrooke, Sherbrooke, Québec, Canada | |
关键词: Fetus; Intestine; Apo B; Apo A-I; Chylomicron; mRNA editing; | |
DOI : 10.1016/0014-5793(96)00896-4 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We investigated insulin's effect on intestinal lipid transport and, particularly, the biogenesis of apolipoproteins crucial to lipoprotein secretion. Adding insulin (3 mU) to the serum-free medium of cultured jejunal explants from human fetuses (17–20 weeks) reduced triglyceride and chylomicron production and inhibited apo B-48 and apo B-100 secretion. When apo B mRNA was assayed by RT-PCR and its editing by primer extension, no change was detectable following the addition of insulin. HDL lipid content, apo A-1 synthesis and RNA level were unaffected by insulin. Collectively, these results suggest that the insulin-stimulated decline in intestinal chylomicron output may involve apo B co- or post-translational modifications.
【 授权许可】
Unknown
【 预 览 】
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