Insulin modulation of newly synthesized apolipoproteins B‐100 and B‐48 in human fetal intestine: Gene expression and mRNA editing are not involved

FEBS Letters

Sinnett, Daniel¹  Nguyen, Thanh Dung¹  Delvin, Edgard¹  Ménard, Daniel²  Thibault, Louise¹  Levy, Emile¹ 

[1] Departments of Nutrition, Pediatrics and Biochemistry, Centre de Recherche, Hôpital Sainte-Justine, Université de Montréal, 3175 Côte Ste-Catherine Road, Montreal, Québec H3T 1C5, Canada;Groupe de Recherche en Biologie de Développement, Département d'Anatomie et de Biologie Cellulaire, Faculté de Médecine, Université de Sherbrooke, Sherbrooke, Québec, Canada

关键词: Fetus;    Intestine;    Apo B;    Apo A-I;    Chylomicron;    mRNA editing;   

DOI  :  10.1016/0014-5793(96)00896-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated insulin's effect on intestinal lipid transport and, particularly, the biogenesis of apolipoproteins crucial to lipoprotein secretion. Adding insulin (3 mU) to the serum-free medium of cultured jejunal explants from human fetuses (17–20 weeks) reduced triglyceride and chylomicron production and inhibited apo B-48 and apo B-100 secretion. When apo B mRNA was assayed by RT-PCR and its editing by primer extension, no change was detectable following the addition of insulin. HDL lipid content, apo A-1 synthesis and RNA level were unaffected by insulin. Collectively, these results suggest that the insulin-stimulated decline in intestinal chylomicron output may involve apo B co- or post-translational modifications.

【 授权许可】

Unknown