Assignment of protein disulphides by a computer method using mass spectrometric data

FEBS Letters

Buonocore, Vincenzo²  Caporale, Carlo²  Caruso, Carla²  Pucci, Piero¹  Sepe, Ciro² 

[1] Dipartimento di Chimica Organica e Biologica, Università Federico II di Napoli and Servizio di Spettrometria di Massa del CNR, Napoli, Italy;Dipartimento di Agrobiologia ed Agrochimica, Università della Tuscia, Via S. Camillo de Lellis, 01100 Viterbo, Italy

关键词: Disulfide bridge;    Protein structure;    Computer method;    Mass spectrometry;   

DOI  :  10.1016/0014-5793(96)00894-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We designed a computer program for the assignment of protein disulphides using mass spectrometric data. All the theoretical linear peptides containing from one to three cysteines are generated on the basis of the protein sequence. Their combination ways are determined in order to create all the possible disulphide-bridged fragments containing from two to six cysteines and to calculate their molecular weight. One, two and three S-S bridges per linked fragment were considered, taking into account the possibility that the fragments contain unabridged residues. The mass data obtained from the spectral analysis of peptide mixtures of the digested protein are then associated to the fitting structures of disulphide-bridged peptides, giving rise to the primary output. This output can then be screened by using information on the specificity of the proteolytic agent(s) used and/or any further mass data provided by Edman degradation and/or carboxypeptidase treatment of the peptide mixtures. The need for such a computer aid is discussed and examples of application are given.

【 授权许可】

Unknown