Human immunodeficiency virus type 1 Nef protein on the cell surface is cytocidal for human CD4+ T cells

FEBS Letters

Adachi, Akio¹  Otake, Kaori²  Fujii, Yoichi²  Tashiro, Masato³ 

[1] Department of Virology, Tokushima University School of Medicine, 3-18-15 Kuramoto-cho, Tokushima 770, Japan;Institute for Laboratory Animal Research, Nagoya University School of Medicine, Nagoya 466, Japan;Department of Virology 1, National Institute of Health, Tokyo 162, Japan

关键词: HIV-1;    Nef;    Cytotoxicity;   

DOI  :  10.1016/0014-5793(96)00862-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have previously shown that the carboxyl-terminal region of human immunodeficiency virus type 1 (HIV-1) Nef antigen present on the outer surface of virus-infected cells has affinity for uninfected T cells. Here, the in vitro cytotoxic potential of HIV-1 Nef on the T cell surface against CD4+ T cells was investigated in detail. Human T cells expressing Nef on the cell surface by transfection with non-infectious mutant HIV-1 proviruses were demonstrated to kill CD4+ T cells effeciently. Furthermore, it was shown that the carboxyl-terminal portion of Nef was cytotoxic for CD4+ T cells and that monoclonal antibody against the carboxyl-terminal region of Nef inhibited Nef induced-cytolysis. Thus, we concluded that Nef protein on CD4+ T cells may play an important role in the specific loss of CD4+ T lymphocytes during HIV-1 infection.

【 授权许可】

Unknown