Soluble Nef antigen of HIV‐1 is cytotoxic for human CD4+ T cells

FEBS Letters

Adachi, Akio¹  Otake, Kaori²  Fujii, Yoichi²  Tashiro, Masato³ 

[1] Department of Virology, Tokushima University School of Medicine, Tokushima 770, Japan;Institute for Laboratory Animal Research, Nagoya University School of Medicine, Nagoya 466, Japan;Department of Virology 1, National Institute of Health, Tokyo 162, Japan

关键词: AIDS;    HIV-1;    Nef;    CD4+ T cell;    Cytotoxicity;   

DOI  :  10.1016/0014-5793(96)00859-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have previously shown that Nef-gene 10 fusion protein induces marked growth arrest of human primary CD4+ T cells. Here, in vitro cytostatic and cytotoxic activities of human immunodeficiency virus type 1 (HIV-1) Nef against CD4+ T cells were extensively investigated. Growth of human CD4+ cells was inhibited significantly just by the addition of purified full-length Nef to cultures. When Nef was cross-linked by anti-Nef antibodies, it became very cytocidal for CD4+ T cells. A high percentage of sera from HIV-1-infected individuals contained soluble Nef. Thus, soluble Nef in vivo may play an important role in immunodysfunction of CD4+ T lymphocytes in HIV-1 infection.

【 授权许可】

Unknown