Immunohistochemical and immunoblotting identification of protein phosphatase 1γ1 in rat salivary glands

FEBS Letters

Takehara, Tadamichi²  Haneji, Tatsuji¹  Kobayashi, Shigeru¹  Nagao, Minako³  Mochizuki, Hajime¹  Shirakawa, Satoko¹  Shima, Hiroshi³ 

[1] Department of Oral Anatomy, Kyushu Dental College, 2-6-1 Manazuru, Kokurakita-ku, Kitakyushu 803, Japan;Department of Preventive Dentistry, Kyushu Dental College, 2-6-1 Manazuru, Kokurakita-ku, Kitakyushu 803, Japan;Carcinogenesis Division, National Cancer Research Institute, Tokyo 104, Japan

关键词: Protein phosphatase;    Salivary gland;    Parotid gland;    Submandibular gland;   

DOI  :  10.1016/0014-5793(96)00821-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have analyzed the distribution of the γ1 isotype of rat protein phosphatase type 1 catalytic subunit in rat salivary glands. Formaldehyde-fixed paraffin sections were reacted with the PP1γ1 antibody using an immunohistochemical method. Positive staining occurred in striated ducts of parotid gland. However, the staining reaction was less intense in submandibular gland. Proteins were also prepared from rat salivary glands and subjected to SDS-PAGE, followed by Western blotting analysis with the PP1γ1 antibody. The antibody interacted with protein corresponding to an estimated molecular mass of 36 kDa present in the parotid gland. The staining reaction was considerably weaker with the proteins from submandibular gland.

【 授权许可】

Unknown