Hybridization of two oligodeoxynucleotides to both strands of an RNA hairpin structure increases the efficiency of RNA‐DNA duplex formation

FEBS Letters

Karpova, Galina²  Malygin, Alexey²  Westermann, Peter¹ 

[1] Cell Biology, Max-Delbrück-Center for Molecular Medicine, 13125 Berlin-Buch, Germany;Novosibirsk Institute of Bio-organic Chemistry, Siberian Division of Russian Academy of Sciences, Lavrentiev pr. 8, Novosibirsk 630090, Russia

关键词: Hybridization;    4.5S RNA;    Escherichia coli;    Oligodeoxynucleotide;    RNA secondary structure;    ON;    oligodeoxynucleotide;    SRP;    signal recognition particle;   

DOI  :  10.1016/0014-5793(96)00798-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hybridization of two oligodeoxyribonucleotides (ON1 and ON2), complementary to opposite strands of the apical domain of Escherichia coli 4.5S RNA, was studied. ON1, complementary to bases 58–71, was not able to form a stable RNA-DNA hybrid whereas ON2, complementary to bases 38–53, was. Addition of both oligonucleotides at the same time resulted in the formation of a ternary complex permitting hybridization of ON1 and increasing hybridization of ON2. Under this condition, binary complexes of ON1 or ON2 with 4.5S RNA were not observed. The data demonstrate that hybridization of oligonucleotides to both strands of an RNA hairpin structure increases the efficiency of hybridization of either oligonucleotide.

【 授权许可】

Unknown