Evidence for bidirectional histamine transport by murine hematopoietic progenitor cells

FEBS Letters

Corbel, Stéphane¹  Dy, Michel¹ 

[1] CNRS URA 1461, Hôpital Necker, 161, rue de Sèvres, 75743 Paris Cedex 15, France

关键词: Hematopoietic progenitor;    Histamine;    Bidirectional transport;    Interleukin-3;    BMC;    bone marrow cells;    αFMH;    α-fluoromethylhistidine;    IL-3;    Interleukin-3;    CFU-S;    colony forming units in spleen;   

DOI  :  10.1016/0014-5793(96)00741-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Murine hematopoietic progenitor cells synthesize substantial amounts of histamine in response to IL-3 or calcium ionophore. They also take up extracellular histamine by an active transport system. In the present study we demonstrate that this system mediates both influx and efflux of histamine. Indeed, MR16155 and thioperamide, the two H₃ antagonists which are most effective in inhibiting histamine uptake, likewise diminish the release of preloaded histamine from bone marrow cells. These compounds also inhibit the release of histamine which has been newly synthesized by hematopoietic progenitors in response to IL-3 or calcium ionophore, as assessed by the accumulation of the mediator inside the cells in the presence of the antagonists. The potency of different histamine receptor antagonists as inhibitors of histamine release increases with their capacity to block histamine uptake.

【 授权许可】

Unknown