Cyclic GMP potentiates phenylephrine but not cyclic ADP‐ribose‐evoked calcium release from rat lacrimal acinar cells

FEBS Letters

Gromada, Jesper¹  Jorgensen, Tino D.²  Dissing, Steen² 

[1] Islet Cell Physiology, Novo Nordisk A/S, Symbion Science Park, Fruebjergvej 3, DK-2100 Copenhagen, Denmark;Department of Medical Physiology, The Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen, Denmark

关键词: Cyclic GMP;    Lacrimal;    Phenylephrine;    Cyclic ADP-ribose;    Inositol trisphosphate;   

DOI  :  10.1016/0014-5793(96)00715-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the present study, we describe a role for cyclic GMP (cGMP) in the signalling pathway that leads from α-adrenergic receptor activation to intracellular Ca²⁺ mobilization in rat lacrimal acinar cells. The α-adrenergic agonist, phenylephrine, stimulates intracellular Ca²⁺ release which is blocked by inhibitors of guanylate cyclase and cGMP-dependent protein kinase Ia. The membrane-permeable cGMP analogues, dibutyryl-cGMP and 8-bromo-cGMP, potentiate (≈5-fold) the Ca²⁺ response to submaximal phenylephrine stimulation. In contrast, the same cGMP analogues have no effect on cyclic ADP-ribose-evoked Ca²⁺ release from permeabilized lacrimal acinar cells. Collectively, these findings suggest that cGMP, via cGMP-dependent protein kinase Iα, is required for intracellular Ca²⁺ release following α-adrenergic receptor activation in lacrimal acinar cells.

【 授权许可】

Unknown