Depletion of intracellular calcium stores activates an outward potassium current in mast and RBL‐1 cells that is correlated with CRAC channel activation

FEBS Letters

Hoth, Markus¹ 

[1] Department of Membrane Biophysics, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg, 37073 Göttingen, Germany

关键词: Store-operated channel;    Calcium release-activated calcium channel;    Potassium conductance;    Barium permeation;    Mast cell;    RBL-1 cell;   

DOI  :  10.1016/0014-5793(96)00673-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Highly Ca²⁺ selective Ca²⁺ channels activated by store depletion have been recently described in several cell types and have been termed CRAC channels (for calcium release-activated calcium). The present study shows that following store depletion in mast and RBL-1 cells, monovalent outward currents could be recorded if the internal solution contained K⁺ but not Cs⁺. The activation of the outward K⁺ current correlated with the activation of I_CRAC, in both time and amplitude, suggesting that the K⁺ current might be carried by CRAC channels. The amplitude of the outward current was increased if external Ca²⁺ was reduced or replaced by external Ba²⁺. The outward K⁺ conductance might have a physiological role in maintaining the driving force for Ca²⁺ entry during the activation of CRAC channels.

【 授权许可】

Unknown