Identification of the functional region on the superantigen Yersinia pseudotuberculosis‐derived mitogen responsible for induction of lymphocyte proliferation by using synthetic peptides

FEBS Letters

Takeda, Tae³  Ishibashi, Makiko³  Yoshino, Ken-ichi³  Shimonishi, Yasutsugu²  Takao, Toshifumi²  Samejima, Yuji¹ 

[1] The Institute of Medical Chemistry, Hoshi University, Shinagawa, Tokyo-142, Japan;Institute for Protein Research, Osaka University, Suita, Osaka-565, Japan;Department of Infectious Diseases Research, National Children's Medical Research Center, Taishido 3-5-31, Setagaya, Tokyo-154, Japan

关键词: Superantigen;    YPM;    Structure-function relationship;    Lymphocyte proliferation;    Yersinia pseudotuberculosis;   

DOI  :  10.1016/0014-5793(96)00656-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Yersinia pseudotuberculosis-derived mitogen (YPM) is the unique Gram-negative bacillary superantigen known. In order to identify the regions on the YPM molecule involved in its superantigenic activity, seven overlapping peptides of the entire YPM molecule were synthesized and tested to evaluate their effects on the YPM-induced proliferation of human peripheral blood lymphocytes. A peptide corresponding to the N-terminal amino acid sequence (1–23) was found to inhibit YPM-induced lymphocyte proliferation in a concentration-dependent manner. The N-terminal peptide was found to show no inhibition of the proliferation induced by the other superantigen (staphylococcal enterotoxin B) or the other T-cell mitogen pertussis toxin, indicating that the inhibition is specific to YPM-induced proliferation. Thus, we have identified the N-terminal region (1–23) of the YPM as one of the functional regions responsible for its superantigenic activity.

【 授权许可】

Unknown