Purification and characterization of a novel superantigen produced by a clinical isolate of Yersinia pseudotuberculosis

FEBS Letters

Takeda, Tae¹  Murata, Hiroshi²  Yoshino, Ken-ichi¹  Shimonishi, Yasutsugu²  Takao, Toshifumi²  Kohsaka, Takao³  Abe, Jun³ 

[1] Department of Infectious Diseases Research, National Children's Medical Research Center, Taishido 3-35-31, Setagaya-ku, Tokyo 154, Japan;Institute for Protein Research, Osaka University, Suita, Osaka 565, Japan;Department of Allergy and Immunology, National Children's Medical Research Center, Setagaya-ku, Tokyo 154, Japan

关键词: Superantigen;    Purification;    Mitogen;    N-terminal sequence;    ESI-MS;    Yersinia pseudotuberculosis;    ESI-MS;    electrospray ionization mass spectrometry;    HPIEC;    high-performance ion-exchange chromatography;    PBMC;    peripheral blood mononuclear cells;    RP-HPLC;    reversed-phase high-performance liquid chromatography;    SE;    staphylococcal enterotoxin;    TCR;    T-cell receptor;    TFA;    trifluoroacetic acid;    TSST-1;    toxic shock syndrome toxin-1;    YPM;    Yersinia pseudotuberculosis-derived mitogen;    Vβ;    β chain variable region;   

DOI  :  10.1016/0014-5793(94)01249-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A superantigen designated as Yersinia pseudotuberculosis-derived mitogen (YPM) was purified in an equal manner from both the culture supernatant and cell lysate of a clinical isolate (KUR-1) of Y. pseudotuberculosis serotype 4b. A significant proliferative response of human peripheral blood mononuclear cells to purified YPM was detectable even at a concentration of 1 pg/ml. The N-terminal sequence of YPM which included 23 amino acid residues was determined, by automated Edman degradation, as Thr-Asp-Tyr-Asp-Asn-Thr-Leu-Asn-Ser-Ile-Pro-Ser-Leu-Arg-Ile-Pro-Asn-Il e-Ala-Thr-Tyr-Thr-Gly-. This sequence differed from not only all the, hitherto, reported superantigens but also known proteins. While molecular weights of known bacterial superantigens are more than 22,000, electrospray ionization mass spectrometry showed that the molecular weight of YPM was 14524.4. These results indicate that YPM comprises a novel superantigen with substantial structural differences from other bacterial superantigens produced by Gram-positive cocci.

【 授权许可】

Unknown