Up‐regulation of cyclooxygenase‐2 mRNA in the rat spinal cord following peripheral inflammation

FEBS Letters

Geisslinger, Gerd¹  Scheuerer, Stefan¹  Brune, Kay¹  Beiche, Flora¹  Goppelt-Struebe, Margarete² 

[1] Department of Experimental and Clinical Pharmacology and Toxicology, University of Erlangen-Nürnberg, Universitätsstr. 22, D-91054 Erlangen, Germany;Department of Medicine IV, University of Erlangen-Nürnberg, Loschgestr. 8, D-91054 Erlangen, Germany

关键词: Cyclooxygenase-2;    Inflammation;    Spinal cord;    RT-PCR;    bp;    basepair;    CFA;    complete Freund's adjuvant;    CNS;    central nervous system;    COX;    cyclooxygenase;    GAPDH;    glyceraldehyde-3-phosphate dehydrogenase;    kb;    kilobase;    MOPS;    4-morpholinepropanesulfonic acid;    NSAIDs;    non-steroidal anti-inflammatory drugs;    PG;    prostaglandin;    RT-PCR;    reverse transcription-polymerase chain reaction;    SSC;    saline sodium citrate;   

DOI  :  10.1016/0014-5793(96)00604-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Prostaglandins (PG) have been described as mediators in spinal nociceptive processing after peripheral inflammation. Enzymes essential for PG biosynthesis, cyclooxygenase isozymes COX-1 and COX-2, have not yet been investigated in the spinal cord. In two studies on rats with adjuvant-induced peripheral inflammation levels of mRNA expression of both COX isoforms were analyzed in the lumbar section of the spinal cord using reverse transcription-polymerase chain reaction (RT-PCR) technique. We could show that mRNA of both COX isoforms is expressed constitutively in the spinal cord with COX-2 as the predominant isoform. Six hours after induction of peripheral inflammation, levels of COX-2 mRNA expression were raised significantly in respect to untreated control rats and returned to baseline within 3 days after induction of inflammation. COX-2 might therefore be regarded as the COX isozyme responsible for spinal PG release in nociceptive processing under a peripheral inflammatory stimulus.

【 授权许可】

Unknown