Prevention of necrosis and activation of apoptosis in oxidatively injured human myeloid leukemia U937 cells

FEBS Letters

Azzi, Angelo²  Cantoni, Orazio¹  Palomba, Letizia¹  Sestili, Piero¹  Cattabeni, Flaminio¹ 

[1] Istituto di Farmacologia e Farmacognosia and Centro di Farmacologia Oncologica Sperimentale, Università di Urbino, Via S. Chiara 27, 61029 Urbino, Italy;Institut für Biochemie und Molekularbiologie der Universität Bern, Bern, Switzerland

关键词: Hydrogen peroxide;    Poly(ADP-ribose)polymerase;    Necrosis;    Apoptosis;    DNA fragmentation;    PBS;    phosphate-buffered saline;    PARP;    poly(ADP-ribose)polymerase;    3AB;    3-aminobenzamide;    FIGE;    field inversion gel electrophoresis;   

DOI  :  10.1016/0014-5793(96)00634-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A 3 h exposure to 1 mM H₂O₂ followed by 6 h post-challenge growth in peroxide-free medium induces necrosis in U937 cells. Addition of the poly(ADP-ribose)polymerase inhibitor 3-aminobenzamide during recovery prevents necrosis and triggers apoptosis, as shown by the appearance of apoptotic bodies, extensive blebbing and formation of multimeric DNA fragments as well as 50 kb double stranded DNA fragments. Thus, the same initial damage can be a triggering event for both apoptotic and necrotic cell death. Furthermore, necrosis does not appear to be a passive response to overwhelming damage.

【 授权许可】

Unknown