Characterization of an Arabidopsis thaliana cDNA encoding an S‐adenosylmethionine‐sensitive threonine synthase Threonine synthase from higher plants

FEBS Letters

Curien, Gilles¹  Dumas, Renaud¹  Ravanel, Stéphane¹  Douce, Roland¹ 

[1] Laboratoire Mixte, Centre National de la Recherche Scientifique/Rhône-Poulenc UMR-41, Rhône-Poulenc Agrochimie, 14-20 rue Pierre Baizet, 69 263 Lyon, France

关键词: Threonine synthase;    o-Phospho-l-homoserine;    S-adenosylmethionine;    Pyridoxal 5′-phosphate;    Chloroplast;    Arabidopsis thaliana;    DTT;    dithiothreitol;    IPTG;    isopropyl β-d-thiogalactopyranoside;    OPA;    o-phthaldialdehyde;    OPH;    o-phospho-l-homoserine;    PLP;    pyridoxal 5′-phosphate;    SAM;    S-adenosylmethionine;   

DOI  :  10.1016/0014-5793(96)00633-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An Arabidopsis thaliana cDNA encoding an Sadenosylmethionine-sensitive threonine synthase (EC 4.2.99.2) has been isolated by functional complementation of an Escherichia coli mutant devoid of threonine synthase activity. Threonine synthase from A. thaliana was shown to be synthesized with a transit peptide. The recombinant protein is activated by Sadenosylmethionine in the same range as the plant threonine synthase and evidence is presented for an involvement of the N-terminal part of the mature enzyme in the sensitivity to Sadenosylmethionine.

【 授权许可】

Unknown