Uncleaved env gp160 of human immunodeficiency virus type 1 is degraded within the Golgi apparatus but not lysosomes in COS‐1 cells

FEBS Letters

Fujisawa, Jun-ichi¹  Kimura, Tominori¹  Nishikawa, Masao¹ 

[1] Department of Microbiology, Kansai Medical University, Moriguchi, Osaka 570, Japan

关键词: HIV-1;    env gp160;    Degradation;    Golgi apparatus;    HIV-1;    human immunodeficiency virus type 1;    PBLs;    peripheral blood lymphocytes;    endo-H;    endoglycosidase H;    ER;    endoplasmic reticulum;    D10;    Dulbecco's modified Eagle's MEM supplemented with 10% heat-inactivated fetal calf serum;    CHX;    cycloheximide;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;    hEGF;    human epidermal growth factor;    MEM/BSA;    Eagle's MEM containing 0.1% BSA;    TRITC;    tetramethylrhodamine isothiocyanate;    FITC;    fluorescein isothiocyanate;    WGA;    wheat germ agglutinin;    VATPase;    vacuolar type H+-ATPase;    BMA;    bafilomycin A1;    Leu;    leupeptin;   

DOI  :  10.1016/0014-5793(96)00614-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The fate of newly synthesized human immunodeficiency virus type 1 env gp160 was examined in COS-1 cells. The results of morphological chase experiments involving cycloheximide demonstrated that gp160 was retained in the Golgi apparatus for longer than the half-life of the molecule. The degradation of gp160 was insensitive to both bafilomycin A₁ and leupeptin (<0.2 mM), which block lysosomal proteolysis. However, degradation was effectively suppressed by leupeptin at higher concentrations, maximally at 1.7 mM. Furthermore, undegraded gp160 was accumulated in the Golgi apparatus, but was not detected in lysosomes. These results indicate that in COS-1 cells gp160 is not degraded in lysosomes, but rather that degradation takes place in the Golgi apparatus.

【 授权许可】

Unknown