FEBS Letters | |
Nitric oxide oxidises a ferrous mammalian lipoxygenase to a pre‐activated ferric species | |
Mäder, Karsten3  Wiesner, Rainer1  Stöβer, Reinhard4  Kühn, Hartmut1  Holzhütter, Hermann-Georg1  Rathmann, Jörg1  Nolting, Hans2  | |
[1] Institute of Biochemistry, University Clinics Charité, Humboldt University, Hessische Str. 3-4, 10115 Berlin, Germany;EMBL Outstation, c/o Deutsches Elektronsynchrotron DESY, Hamburg, Germany;Department of Pharmacy, Humboldt University, Berlin, Germany;Department of Chemistry, Humboldt University, Berlin, Germany | |
关键词: X-ray absorption spectroscopy; Inflammation; Free radical; Eicosanoid; | |
DOI : 10.1016/0014-5793(96)00591-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Nitric oxide is known as an inhibitor of soybean lipoxygenase-1. Investigating the interaction of a mammalian 15-lipoxygenase with nitric oxide, we found that this enzyme is also inhibited reversibly when incubated with nitric oxide for a short time period (5 s) under anaerobic conditions. This inhibition may be due to the formation of a dissociable lipoxygenase-nitric oxide complex. With longer incubation periods the ferrous lipoxygenase is oxidised to a ferric form. This oxidation renders the enzyme more susceptible to peroxide activation, causing a time-dependent shortening of the kinetic lag phase.
【 授权许可】
Unknown
【 预 览 】
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