Nitric oxide oxidises a ferrous mammalian lipoxygenase to a pre‐activated ferric species

FEBS Letters

Mäder, Karsten³  Wiesner, Rainer¹  Stöβer, Reinhard⁴  Kühn, Hartmut¹  Holzhütter, Hermann-Georg¹  Rathmann, Jörg¹  Nolting, Hans² 

[1] Institute of Biochemistry, University Clinics Charité, Humboldt University, Hessische Str. 3-4, 10115 Berlin, Germany;EMBL Outstation, c/o Deutsches Elektronsynchrotron DESY, Hamburg, Germany;Department of Pharmacy, Humboldt University, Berlin, Germany;Department of Chemistry, Humboldt University, Berlin, Germany

关键词: X-ray absorption spectroscopy;    Inflammation;    Free radical;    Eicosanoid;   

DOI  :  10.1016/0014-5793(96)00591-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nitric oxide is known as an inhibitor of soybean lipoxygenase-1. Investigating the interaction of a mammalian 15-lipoxygenase with nitric oxide, we found that this enzyme is also inhibited reversibly when incubated with nitric oxide for a short time period (5 s) under anaerobic conditions. This inhibition may be due to the formation of a dissociable lipoxygenase-nitric oxide complex. With longer incubation periods the ferrous lipoxygenase is oxidised to a ferric form. This oxidation renders the enzyme more susceptible to peroxide activation, causing a time-dependent shortening of the kinetic lag phase.

【 授权许可】

Unknown