Role of ascorbic acid in the metabolism of S‐nitroso‐glutathione

FEBS Letters

Inoue, Masayasu¹  Yamaguchi, Makiko¹  Kashiba-Iwatsuki, Misato¹ 

[1] Department of Biochemistry, Osaka City University Medical School, 1-4-54 Asahi-machi, Aheno, Osaka 545, Japan

关键词: S-Nitroso-glutathione;    Ascorbic acid;    Nitric oxide;    Thiol abr|NO;    nitric oxide;    RS-NO;    S-nitroso-thiol;    GS-NO;    S-nitroso-glutathione;    EDRF;    endothelium-derived relaxing factor;    DTNB;    5;    5′-dithiobis(2-nitrobenzoic acid);    NOx−;    nitrite and nitrate;    GSH;    reduced form glutathione;    GSSG;    oxidized form glutathione;    ESR;    electron spin resonance;    LPS;    lipopolysaccharide;    DETAPAC;    diethylenetriamine-N;    N;    N′;    N″;    N″-pentaacetic acid;   

DOI  :  10.1016/0014-5793(96)00560-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Although nitric oxide (NO) has been known to generate S-nitroso-thiols (RSNOs), dynamic aspects of their metabolism remain to be elucidated. The present work reports the reactivity of S-nitroso-glutathione (GS-NO) with various compounds with reducing activity. Kinetic analysis revealed that among various reagents tested, ascorbic acid showed a potent activity to decompose GS-NO to glutathione and oxidized products of NO. During the reaction of GS-NO and ascorbic acid, monodehydroascorbate was found to appear as an intermediate. These results suggest that ascorbic acid might be an important modulator for RS-NO metabolism.

【 授权许可】

Unknown