FEBS Letters | |
Role of ascorbic acid in the metabolism of S‐nitroso‐glutathione | |
Inoue, Masayasu1  Yamaguchi, Makiko1  Kashiba-Iwatsuki, Misato1  | |
[1] Department of Biochemistry, Osaka City University Medical School, 1-4-54 Asahi-machi, Aheno, Osaka 545, Japan | |
关键词: S-Nitroso-glutathione; Ascorbic acid; Nitric oxide; Thiol abr|NO; nitric oxide; RS-NO; S-nitroso-thiol; GS-NO; S-nitroso-glutathione; EDRF; endothelium-derived relaxing factor; DTNB; 5; 5′-dithiobis(2-nitrobenzoic acid); NOx−; nitrite and nitrate; GSH; reduced form glutathione; GSSG; oxidized form glutathione; ESR; electron spin resonance; LPS; lipopolysaccharide; DETAPAC; diethylenetriamine-N; N; N′; N″; N″-pentaacetic acid; | |
DOI : 10.1016/0014-5793(96)00560-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Although nitric oxide (NO) has been known to generate S-nitroso-thiols (RSNOs), dynamic aspects of their metabolism remain to be elucidated. The present work reports the reactivity of S-nitroso-glutathione (GS-NO) with various compounds with reducing activity. Kinetic analysis revealed that among various reagents tested, ascorbic acid showed a potent activity to decompose GS-NO to glutathione and oxidized products of NO. During the reaction of GS-NO and ascorbic acid, monodehydroascorbate was found to appear as an intermediate. These results suggest that ascorbic acid might be an important modulator for RS-NO metabolism.
【 授权许可】
Unknown
【 预 览 】
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