Interaction of nitrogen dioxide with human plasma Antioxidant depletion and oxidative damage

FEBS Letters

Tarkington, Brian K.¹  Motchnik, Paul³  Duvall, Timothy R.¹  Cross, Carroll E.²  Hu, Miao-Lin²  Louie, Samuel²  Halliwell, Barry² 

[1] Research Center, UC Davis, Davis, CA 95616, USA;Pulmonary-Critical Care Medicine, U.C. Davis Medical Center, 4301 X Street, Sacramento, CA 95817, USA;Division of Biochemistry and Molecular Biology, Barker Hall, UC Berkely, CA 94720, USA

关键词: Nitrogen dioxide;    Lipid peroxidation;    Ascorbic acid;    Nitric oxide;    Uric acid;    Antioxidant;   

DOI  :  10.1016/0014-5793(92)81185-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nitrogen dioxide (NO*₂) is often present in inhaled air and may be generated in vivo from nitric oxide. Exposure of human blood plasma to NO*₂ caused rapid losses of ascorbic acid, uric acid and protein thiol groups, as well as lipid peroxidation and depletions of α-tocopherol, bilirubin and ubiquinol-10. No increase in protein carbonyls was detected. Supplementation of plasma with ascorbate decreased the rates of lipid peroxidation. α-tocopherol depletion and loss of uric acid. Uric acid supplementation decreased rates of lipid peroxidation but not the loss of α-tecopherol. We conclude that ascorbic acid, protein -SH groups, uric acid and α-tocopherol may be important agents protecting against NO*₂ in vivo. If these antioxidants are depleted, peroxidation of lipids occurs and might contribute to the toxicity of NO*₂.

【 授权许可】

Unknown