Genomic simple repetitive DNAs are targets for differential binding of nuclear proteins

FEBS Letters

Kyas, Andreas¹  Epplen, Jörg T.¹  Mäueler, Winfried¹ 

[1] Molecular Human Genetics, MA5, Ruhr-University, 44780 , Germany

关键词: Composite simple repeat;    DNA secondary structure;    Microsatellite;    Polypurine;    H-DNA configuration;    Z-DNA configuration;   

DOI  :  10.1016/0014-5793(96)00526-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The biological meaning of abundant simple repetitive DNA sequences in eukaryote genomes is obscure. Therefore, (GAA) _n , (GT) _n , and composite (GT) _n (GA) _m blocks were characterized for protein binding in the repeat and flanking sequences of cloned genomic DNA fragments. In gel mobility shift and competition assays the binding of nuclear proteins to the repeats was specific (including some flanking single copy sequences). DNase footprinting revealed the target sequences within and adjacent to the repeats. Chemical modifications (Os0₄, DAPC) DAmonstrated non-B DNA structures in the polypurine blocks. The binding of nuclear proteins in and around simple repeat sequences refute biological insignificance of all of these ubiquitously interspersed elements.

【 授权许可】

Unknown