Footprinting of DNA secondary structure by high‐intensity (laser) ultraviolet irradiation

FEBS Letters

Kovalsky, O.I.²  Budowsky, E.I.²  Rubin, L.B.¹  Simukova, N.A.²  Yakovlev, D.Yu.² 

[1] Institute of Nuclear Physics, Moscow State University, Moscow, USSR;Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, GSP7, Miklukho-Maklaya str. 16/10,117871 Moscow, USSR

关键词: Footprinting;    DNA secondary structure;    DNA splitting;    Laser ultraviolet irradiation;    Plasmid pBR 322;   

DOI  :  10.1016/0014-5793(85)80894-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The action of high-intensity ultraviolet pulse laser radiation on a 161 bp fragment of pBR 322 DNA (EcoRI-Msp I fragment) was studied. At doses up to 5 × 10¹⁸ photons/cm² the N-glycosidic bond splitting is negligible. The action of hot piperidine on irradiated DNA leads to chain splitting at the residues, modified via biphotonic processes. The modification and, hence, splitting efficiences depend on the type of base (G>T>A>C) and on its position in the sequence. Preferentially modified bases in the opposite strands of double-stranded DNA belong, mainly, to the same or adjacent base pairs. Residues in the Pribnow box are modified considerably less, than in the sequences, immediately upstream and downstream. This approach seems to be useful in footprinting of DNA secondary structure peculiarities and alterations, conjugated with the functional role and state of the respective fragment.

【 授权许可】

Unknown