Immunolocalization of an inwardly rectifying K+ channel, KAB‐2 (Kir4.1), in the basolateral membrane of renal distal tubular epithelia

FEBS Letters

Isomoto, Shojiro⁴  Ito, Hiroyuki²  Horio, Yoshiyuki⁴  Tonosaki, Akira³  Inanobe, Atsushi²  Kurachi, Yoshihisa²  Mori, Keiji³  Tomoike, Hitonobu¹  Hibino, Hiroshi⁴  Ito, Minoru² 

[1] the First Department of Internal Medicine, Yamagata University School of Medicine, Yamagata 990-23, Japan;Department of Cell Biology and Signaling, Yamagata University School of Medicine, Yamagata 990-23, Japan;Department of Anatomy, Yamagata University School of Medicine, Yamagata 990-23, Japan;Department of Pharmacology II, Faculty of Medicine, Osaka University, 2-2 Yamadaoka, Suita 565, Osaka, Japan

关键词: Potassium channel;    Basolateral membrane;    Kidney tubule;    distal;    Immunohistochemistry;    Immunogold electron microscopy;   

DOI  :  10.1016/0014-5793(96)00502-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Immunolocalization of K_AB−2 (Kir4.1), an inwardly ectifying K⁺ channel with a putative ATP-binding domain, was examined in rat kidney where expression of K_AB−2 mRNA was previously shown. Anti-K_AB−2 antibody was raised in rabbit and then affinity-purified. An immunohistochemical study revealed that K_AB-2 immunoreactivity was detected specifically in the basolateral membrane of distal tubular epithelia. Therefore, K_AB-2 is the first K⁺ channel shown to be localized in the basolateral membrane of renal epithelia. The finding suggests that K_AB-2 may contribute to supplying K⁺ to the Na⁺-K⁺ pump, which is abundant in the basolateral membrane of distal tubular epithelia, as well as to maintenance of the deep negative membrane potential of these cells.

【 授权许可】

Unknown