FEBS Letters | |
Kir2.2v: a possible negative regulator of the inwardly rectifying K+ channel Kir2.2 | |
Namba, Noriyuki3  Seino, Susumu3  Seino, Yoshiki1  Gonoi, Tohru2  Inagaki, Nobuya3  | |
[1] Department of Pediatrics, Okayama University Medical School, Shikata-cho, Okayama 700, Japan;Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1, Inohana, Chuo-ku, Chiba 260, Japan;Division of Molecular Medicine, Center for Biomedical Science, Chiba University, School of Medicine, 1-8-1, Inohana, Chuo-ku, Chiba 260, Japan | |
关键词: Inwardly rectifying K+ channel; Gene; Heteromultimer; Xenopus oocytes; Site-directed mutagenesis; Chimera; | |
DOI : 10.1016/0014-5793(96)00445-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We have cloned the human genes encoding the inwardly rectifying K+ (Kir) channel subunits, Kir2.2 (hKir2.2) and its variant, termed hKir2.2v. When expressed in Xenopus oocytes, hKir2.2 produced strong inwardly rectifying K+ currents, whereas the expression of hKir2.2v did not elicit significant currents. Coexpression of hKir2.2v with hKir2.2 showed an hKir2.2v inhibition of hKir2.2 K+ currents, indicating that it acts as a negative regulator of hKir2.2 channel activity. Mutational analysis of hKir2.2v and studies of chimeras between hKir2.2 and hKir2.2v suggest that the intracellular C-terminal region of hKir2.2v participates in the negative regulation of the hKir2.2v channel activity.
【 授权许可】
Unknown
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