Kir2.2v: a possible negative regulator of the inwardly rectifying K+ channel Kir2.2

FEBS Letters

Namba, Noriyuki³  Seino, Susumu³  Seino, Yoshiki¹  Gonoi, Tohru²  Inagaki, Nobuya³ 

[1] Department of Pediatrics, Okayama University Medical School, Shikata-cho, Okayama 700, Japan;Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1, Inohana, Chuo-ku, Chiba 260, Japan;Division of Molecular Medicine, Center for Biomedical Science, Chiba University, School of Medicine, 1-8-1, Inohana, Chuo-ku, Chiba 260, Japan

关键词: Inwardly rectifying K+ channel;    Gene;    Heteromultimer;    Xenopus oocytes;    Site-directed mutagenesis;    Chimera;   

DOI  :  10.1016/0014-5793(96)00445-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have cloned the human genes encoding the inwardly rectifying K⁺ (Kir) channel subunits, Kir2.2 (hKir2.2) and its variant, termed hKir2.2v. When expressed in Xenopus oocytes, hKir2.2 produced strong inwardly rectifying K⁺ currents, whereas the expression of hKir2.2v did not elicit significant currents. Coexpression of hKir2.2v with hKir2.2 showed an hKir2.2v inhibition of hKir2.2 K⁺ currents, indicating that it acts as a negative regulator of hKir2.2 channel activity. Mutational analysis of hKir2.2v and studies of chimeras between hKir2.2 and hKir2.2v suggest that the intracellular C-terminal region of hKir2.2v participates in the negative regulation of the hKir2.2v channel activity.

【 授权许可】

Unknown