Possible nystatin‐protein interaction in yeast plasma membrane vesicles in the presence of ergosterol. A Förster energy transfer study

FEBS Letters

Konopásek, Ivo³  Kvasnička, Peter⁴  Opekarová, Miroslava⁵  Strzalka, Kazimierz¹  Amler, Evžen²  Urbanová, Petra²  Sigler, Karel⁵ 

[1] Jagellonian University, The Jan Zurzycki Institute of Molecular Biology, Al. Mickiewicza 3, 31 120 Krakow, Poland;Institute of Physiology, CAS, Vídeňská 1083, 142 20 Prague 4, Czech Republic;Charles University, Faculty of Science, Viničná 5, 128 44 Prague 2, Czech Republic;Comenius University, Faculty of Mathematics and Physics, Mlynská dolina, Bratislava, Slovak Republic;Institute of Microbiology, Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague 4, Czech Republic

关键词: Nystatin;    Yeast plasma membrane vesicle;    Arginine transport;    Förster energy transfer;    Tryptophan fluorescence;    Fluorescence lifetime;    FRET;    Förster energy transfer;    PL;    E coli phospholipid liposomes;    PE;    ergosterol-containing E coli liposomes;    PLM;    yeast membranes fused with the phospholipid liposomes;    PEM;    membranes fused with ergosterol-containing liposomes;    χ R 2 the reduced chi-square;    FWHM;    full-width of the half-maximum;   

DOI  :  10.1016/0014-5793(96)00434-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Förster energy transfer from tryptophan residues of membrane proteins to nystatin was measured in reconstituted yeast plasma membrane vesicles free of, or doped with, ergosterol. We wanted to elucidate whether the functional change of membrane transport proteins from H⁺ symporters to facilitators, observed in ergosterol-containing plasma membrane vesicles on addition of nystatin [Opekarová and Tanner (1994) FEBS Lett. 350, 46–50], is reflected in altered protein-nystatin relations within the membrane. Both frequency-domain and time-domain time-resolved fluorescence spectroscopy showed that in the presence of ergosterol nystatin is located much closer to membrane proteins than in its absence.

【 授权许可】

Unknown