Triplex‐mediated cleavage of DNA by 1,10‐phenanthroline‐linked 2′‐O‐methyl RNA

FEBS Letters

Morioka, Hiroshi¹  Inoue, Hideo¹  Ohtsuka, Eiko¹  Shimizu, Miho¹ 

[1] Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060, Japan

关键词: 2′-O-Methyl RNA;    Triple helix;    1;    10-Phenanthroline;    Human thrombomodulin gene;    Chemical cleavage;    A;    2′-deoxyadenosine;    G;    2′-deoxyguanosine;    C;    2′-deoxycytidine;    T;    thymidine;    M;    5-methyl-2′-deoxycytidine;    Um;    2′-O-methyluridine;    Cm;    2′-O-methylcytidine;    TFO;    triple helix forming oligonucleotide;    TM;    thrombomodulin;    OP;    1;    10-phenanthroline;    OP-oligo;    OP-linked oligonucleotide;    ODN;    oligodeoxynucleotide;    MPA;    β-mercaptopropionic acid;    OP-m;    OP-linked 2′-O-methyl RNA;    OP-d;    OP-linked oligodeoxynucleotide;   

DOI  :  10.1016/0014-5793(96)00307-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have previously reported that 2′-O-methyl RNAs are efficient probes for duplex DNA. Here we describe the design, synthesis, and DNA cleaving activity of 1,10-phenanthroline (OP)-linked 2′-O-methyl RNA (OP-m). Although a local triple helix was formed, both with OP-m and a control OP-linked DNA at the target sequence of the duplex DNA, the promoter region of the human thrombomodulin gene, the cleavage efficiencies on both strands were not proportional when OP-m was used as a cleavage agent. These results may reflect the structural differences of the respective triple helices and the duplex-triplex junction, formed from the two types of triplex-forming oligonucleotides, 2′-O-methyl RNA and DNA. Since the OP-ms were found to work as preferential purine-strand cutters for duplex DNA, they would be useful as unique tools for genome analysis.

【 授权许可】

Unknown