The 1,10‐phenanthroline micelles‐copper(I) complex catalyzes protein degradation

FEBS Letters

Kito, Makoto¹  Urade, Reiko¹  Takenaka, Yasuyuki¹ 

[1] Research Institute for Food Science, Kyoto University, Uji, Kyoto 611, Japan

关键词: Artificial protease;    1;    10-Phenanthroline;    Copper(I);    PDI;    protein-disulfide isomerase;    OP;    1;    10-phenanthroline;    bis-Tris buffer;    bis(2-hydroxyethyl) iminotris (hydroxymethyl) methane-HCl;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;    HPLC;    high performance liquid chromatography;   

DOI  :  10.1016/0014-5793(95)00204-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The 1,10-phenanthroline micelles-copper(I) coordinated complex, not the mono-dispersed bis 1,10-phenanthroline-copper(I) one, rapidly degraded proteins in the presence of β-mercaptoethanol in the neutral-to-mild acidic region under aerobic conditions. The degradation products derived from α-casein were peptides and amino acids.

【 授权许可】

Unknown