Transduction signals induced in rat brain cortex astrocytes by the HIV‐1 gp120 glycoprotein

FEBS Letters

Meldolesi, J.¹  Grohovaz, F.¹  Racchetti, G.¹  Codazzi, F.¹ 

[1] Department of Pharmacology and B. Ceccarelli Neurobiology Center, University of Milano; CNR Center of Cellular and Molecular Pharmacology; and DIBIT, San Raffaele Scientific Institute, Via Olgettina 58, 02132 Milan, Italy

关键词: Astrocyte;    gp120;    [Ca2+]i;    Tyrosine phosphorylation;    HIV neurotoxicity;   

DOI  :  10.1016/0014-5793(96)00301-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cultures of rat brain cortex astrocytes were exposed to 10⁻¹⁰−10⁻⁹ M of the HIV-1 envelope glycoprotein, gp120. No specific binding was revealed by the iodinated protein, suggesting expression of only a few sites onto the cells. In contrast, two transduction signals were rapidly induced by gp120: increased tyrosine phosphorylation of a ∼56 kDa protein and increased [Ca²⁺]_i. This latter effect, present in of the investigated astrocytes, consisted in: discrete or biphasic peaks; slowly rising plateaus; and various types of oscillations. Moreover, in apparently unresponsive cells [Ca²⁺]_i rose slowly (45 min) to double the resting levels. Rat brain cortex astrocytes thus appear highly sensitive to gp120. The induced array of signals might contribute to neurotoxicity during HIV infection.

【 授权许可】

Unknown