FEBS Letters | |
Wortmannin inhibits store‐mediated calcium entry and protein tyrosine phosphorylation in human platelets | |
Farndale, Richard W.1  Sage, Stewart O.2  Jenner, Susanne2  | |
[1] Department of Biochemistry, University of Cambridge, Cambridge, CB2 1QW, UK;The Physiological Laboratory, University of Cambridge, Cambridge, CB2 3EG, UK | |
关键词: Thapsigargin; Tyrosine phosphorylation; Store-mediated calcium influx; Wortmannin; Platelet; Fura-2/AM; fura-2 acetoxymethyl ester; WT; wortmannin; [Ca2+]i; intracellular calcium; | |
DOI : 10.1016/0014-5793(96)00130-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The effects of the WT on store-mediated Ca2+ entry and protein tyrosine phosphorylation were investigated in fura-2-loaded human platelets. Wortmannin (2 μM) attenuated the rise in [Ca2+]i caused by Ca2+ entry while having no effect on the mobilisation of Ca2+ from internal stores. It also reduced store-depletion-evoked protein tyrosine phosphorylation. These findings demonstrate that WT is an inhibitor of tyrosine phosphorylation and store-mediated calcium entry and provide further evidence for the involvement of a tyrosine phosphorylation step in the link between Ca2+ store depletion and Ca2+ influx in human platelets.
【 授权许可】
Unknown
【 预 览 】
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