Wortmannin inhibits store‐mediated calcium entry and protein tyrosine phosphorylation in human platelets

FEBS Letters

Farndale, Richard W.¹  Sage, Stewart O.²  Jenner, Susanne² 

[1] Department of Biochemistry, University of Cambridge, Cambridge, CB2 1QW, UK;The Physiological Laboratory, University of Cambridge, Cambridge, CB2 3EG, UK

关键词: Thapsigargin;    Tyrosine phosphorylation;    Store-mediated calcium influx;    Wortmannin;    Platelet;    Fura-2/AM;    fura-2 acetoxymethyl ester;    WT;    wortmannin;    [Ca2+]i;    intracellular calcium;   

DOI  :  10.1016/0014-5793(96)00130-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effects of the WT on store-mediated Ca²⁺ entry and protein tyrosine phosphorylation were investigated in fura-2-loaded human platelets. Wortmannin (2 μM) attenuated the rise in [Ca²⁺]_i caused by Ca²⁺ entry while having no effect on the mobilisation of Ca²⁺ from internal stores. It also reduced store-depletion-evoked protein tyrosine phosphorylation. These findings demonstrate that WT is an inhibitor of tyrosine phosphorylation and store-mediated calcium entry and provide further evidence for the involvement of a tyrosine phosphorylation step in the link between Ca²⁺ store depletion and Ca²⁺ influx in human platelets.

【 授权许可】

Unknown