Ultraviolet‐A induces activation of AP‐1 in cultured human keratinocytes

FEBS Letters

Mergny, Jean-Louis³  Djavaheri-Mergny, Mojgan²  Dubertret, Louis²  Bertrand, France¹  Mazière, Cécile⁴  Santus, René²  Mazière, Jean-Claude² 

[1] Laboratoire de Biologie Cellulaire, INSERM U402, 27 rue Chaligny, 75012 Paris, France;Laboratoire de Dermatologie, INSERM U312, Hôpital Saint-Louis, 1 avenue Claude Vellefaux, 75475 Paris, France;Laboratoire de Biophysique, INSERM U201, CNRS UA481, MNHN, 43 rue Cuvier, 75231 Paris Cedex 05, France;Département de Biochimie, Faculté de Médicine Saint-Antoine, 27 rue Chaligny, 75012 Paris, France

关键词: Ultraviolet A;    Keratinocyte;    Transcription factor AP-1;    Antioxidant;    Lipid peroxidation;    UV;    ultraviolet;    AP-1;    activator protein-1;    Nac;    N-acetylcysteine;    PMA;    phorbol 12-myristate 13-acetate;    MDA;    malondialdeyde;    TBARS;    thiobarbituric acid reactive substances;    EMSA;    electrophoresis mobility shift assay;   

DOI  :  10.1016/0014-5793(96)00294-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

UV-A irradiation induces a time-dependent activation of AP-1 in NCTC 2544 human keratinocytes. 4 h after irradiation, a 2–3-fold increase in AP-1 activity is observed in human keratinocytes and fibroblasts. Activation is still detectable 24 h later. The UV-A induced AP-1 binding complex is shown to contain c-Fos and c-Jun proteins. Lipophilic vitamin E impedes UV-A induced lipid peroxidation but does not prevent AP-1 activation which is inhibited by N-acetylcysteine, a hydrophilic antioxidant. This finding suggests that UV-A-dependent AP-1 activation is sensitive to the cellular redox state but is not related to membrane lipid peroxidation.

【 授权许可】

Unknown