Mitochondrial import of a yeast cytoplasmic tRNALys: possible roles of aminoacylation and modified nucleosides in subcellular partitioning

FEBS Letters

Entelis, Nina S.¹  Krasheninnikov, Igor A.²  Martin, Robert P.¹  Tarassov, Ivan A.¹ 

[1] UPR 9005 du CNRS, ‘Mecanismes Moleculaires de Division Cellulaire et du Developpement’, 15 rue Rene Descartes, 67084 Strasbourg, France;Division of Molecular Biology, Biology Faculty, Moscow State University, Moscow 119899, Russia

关键词: Mitochondrial import;    tRNACUU Lys;    Aminoacylation;    (Saccharomyces cerevisiae);    tRK1;    yeast tRNACUU Lys;    tRK2;    yeast cytoplasmic tRNAU∗UU Lys;    KRS;    yeast cytoplasmic lysyl-tRNA-synthetase;    MSK;    yeast mitochondrial lysyl-tRNA-synthetase;    IDP;    import directing proteins;    MP;    mitochondrial proteins;   

DOI  :  10.1016/0014-5793(96)00259-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The yeast tRNA_CUU ^Lys is transcribed from a nuclear gene and then unequally redistributed between the cytosol (97–98%) and mitochondria (2–3%). We have optimized the conditions for its specific import into isolated mitochondria. However, only a minor fraction (about 0.5%) of the added tRNA was translocated into the organelles. An in vitro transcript, once aminoacylated, appeared to be a better import substrate than the natural tRNA which carries modified nucleosides. The tRNA is translocated across mitochondrial membranes in its aminoacylated form and remains relatively stable inside the organelle. Possible roles of aminoacylation, tRNA-protein interactions and nucleoside modification in subcellular partitioning of the tRNA are discussed.

【 授权许可】

Unknown