Cloning and chromosomal localization of a cDNA encoding a mitochondrial porin from Drosophila melanogaster

FEBS Letters

Caggese, Corrado³  Marino, Mario²  De Pinto, Vito²  Perosa, Federico¹  Caizzi, Ruggiero³  Neri, Mariangela²  Messina, Angela² 

[1] Dipartimento di Scienze Biomediche ed Oncologia Umana - Medicina Interna, Universitá di Bari, pza G. Cesare 1, 70123 Bari, Italy;Istituto di Scienze Biochimiche e Farmacologiche, Facoltà di Scienze M.F.N., Universitá di Catania, viale A. Doria 6, 95125 Catania, Italy;Istituto di Genetica, Facoltà di Scienze M.F.N., Università di Bari, via Amendola 1651A, 70126 Bari, Italy

关键词: Porin pore;    Drosophila melanogaster;    cDNA;    Sequence;    Chromosomal localization;    Mitochondria;   

DOI  :  10.1016/0014-5793(96)00268-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have raised polyclonal antibodies against purified the Drosphila melanogaster mitochondrial porin. They showed high titre and specificity and were thus used as a tool for screening an expression library. The isolated clone 1T1 showed 74% sequence identity in the last 19 residues at the C-terminus of human porin. A subclone of 1T1, containing the porin-like sequence, was thus used as a probe for re-screening a cDNA library and several positive clones were plaque-purified. We present here the sequence of a 1363 bp cDNA encoding a protein of 279 amino acids. Its identity with porin was also confirmed by N-terminal Edman degradation of the purified protein. The D. melanogaster porin shows an overall 51.8% identity with human porin isoform 1 (porin 31HL or HVDAC1) and an overall 55.7% identity with human porin isoform 2 (HVDAC2). Hydrophobicity plots and secondary structure predictions showed a very high similarity with data obtained from known porin sequences. The D. melanogaster porin cDNA was used as a probe for in situ hybridization to polytenic salivar gland chromosomes. It hybridizes with different intensities in two sites, in chromosome 2L, at region 31E and in chromosome 3L at region 79D. Thus, also in Drosophila melanogaster porin polypeptide(s) belong(s) to a multigene family.

【 授权许可】

Unknown