Second messenger up‐regulation of androgen receptor gene transcription is absent in androgen insensitive human prostatic carcinoma cell lines, PC‐3 and DU‐145

FEBS Letters

Stamp, G.W.H.¹  Hurst, H.²  Abel, P.³  Waxman, J.⁴  Lalani, E.-N.¹  Stubbs, A.P.¹ 

[1] Department of Histopathology, Royal Postgraduate Medical School Hammersmith Hospital, Du Cane Road, London W12 0NN, UK;Department of ICRF Oncology Unit, Royal Postgraduate Medical School Hammersmith Hospital, Du Cane Road, London W12 0NN, UK;Department of Surgery, Royal Postgraduate Medical School Hammersmith Hospital, Du Cane Road, London W12 0NN, UK;Department of Oncology, Royal Postgraduate Medical School Hammersmith Hospital, Du Cane Road, London W12 0NN, UK

关键词: Androgen receptor;    Electromobility shift assay;    Prostate cancer;   

DOI  :  10.1016/0014-5793(96)00241-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A theoretical pathway of transcriptional regulation of the androgen receptor (AR) gene is via a cAMP response element (CRE) present in its promoter region (−508 to −501). After 20 h of stimulation with 8-bromo-cAMP, AR mRNA was upregulated in LNCaP but not in either PC-3 or DU-145 cell lines. We have demonstrated that the level of CRE binding protein (CREB) was the same in all cell lines and that the putative AR-CRE forms specific and competable protein interactions with CREB. The ability to regulate AR gene transcription via the second messenger pathway is lost in the PC-3 and DU-145 cell lines. This may be an important primary mechanism of androgen insensitivity in prostate cancer.

【 授权许可】

Unknown