Cloning and sequencing of the 5′ region of the human glucose‐6‐phosphatase gene: transcriptional regulation by cAMP, insulin and glucocorticoids in H4IIE hepatoma cells

FEBS Letters

Allan, Bernard B.¹  Schmoll, Dieter¹  Burchell, Ann¹ 

[1] Department of Obstetrics and Gynaecology, Ninewells Hospital and Medical School, University of Dundee, Dundee, DD1 9SY, UK

关键词: Glucose-6-phosphatase;    Dexamethasone;    Insulin;    Gene regulation;    cAMP;    C/EBP;    CCAAT/enhancer binding protein;    HNF;    hepatocyte nuclear factor;    CRE;    cAMP response element;    IRE;    insulin response element;    GRE;    glucocorticoid response element;   

DOI  :  10.1016/0014-5793(96)00224-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have cloned and sequenced the first 1.2 kb of the 5′ region of the human glucose-6-phosphatase gene. Transfection of H4IIE hepatoma cells with the 1.2 kb fragment fused to a luciferase reporter gene demonstrated both basal and hormone responsive luciferase activity. Dexamethasone increased and insulin decreased luciferase activity. Insulin and dibutyryl cyclic AMP both significantly decreased activity in the presence of dexamethasone.

【 授权许可】

Unknown