Stimulation of human peripheral blood lymphocytes by bioactive peptides derived from bovine milk proteins

FEBS Letters

Meisel, Hans¹  Kayser, Holger² 

[1] Bundesanstalt für Milchforschung, Institut für Chemie und Physik, Hermann-Weigmann-Str. 1, D-24103 Kiel, Germany;Institut für Humanernährung und Lebensmittelkunde der Christian-Albrechts-Universität, Düsternbrooker Weg 17, D-24105 Kiel, Germany

关键词: Bioactive peptide;    Immunopeptide;    Milk protein;    Human peripheral blood lymphocyte;    PBL;    peripheral blood lymphocytes;    BrdU;    5-bromo2′-deoxyuridine;    ConA;    concanavalin A;    TMB;    tetramethylbenzidine;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/0014-5793(96)00207-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The in vitro modulation of the proliferation of human peripheral blood lymphocytes of different synthetic peptides derived from milk proteins was investigated. Therefore, proliferation changes were followed up after incorporation of BrdU into the DNA, and the influence on protein biosynthesis was measured using the [³H]leucine incorporation test. Tyr-Gly and Tyr-Gly-Gly significantly enhanced (maximal 90 and 35%, respectively) the proliferation of PBL. For β-casomorphin-7 and β-casokinin-10, lymphocyte proliferation was suppressed at lower concentrations, but stimulated at higher concentrations (≥10⁻⁷ mol/l). Protein synthesis was stimulated (maxima at 25%) only with Tyr-Gly and Tyr-Gly-Gly. The findings point to a need for further studies on the possible function of peptides derived from milk proteins as orally bioavailable immunopotentiatory compounds.

【 授权许可】

Unknown