Acidic phospholipids inhibit the phospholipase D activity of rat brain neuronal nuclei

FEBS Letters

McCartney, D.G.¹  Singh, I.N.¹  Freysz, L.²  Kanfer, J.N.¹ 

[1] Department of Biochemistry and Molecular Biology, University of Manitoba, Winnipeg, Manitoba R3E 0W3, Canada;Centre de Neurochemie, Strasbourg 67084, France

关键词: Phospholipase D;    Neuronal nuclei;    Phosphatidic acid;    Phosphatidylinositol-4;    5-diphosphate;    Acidic phospholipids;    PLD;    phospholipase D;    PtdCho;    phosphatidylcholine;    PtdSer;    phosphatidylserine;    Ptdglycerol;    phosphatidylglycerol;    PtdE-tOH;    phosphatidylethanol;    PtdA;    phosphatidic acid;    PIP2;    phosphatidylinositol-4;    5-diphosphate;    PtdIns;    phosphatidylinositol;    PLC;    phospholipase C;    ARF;    ADP ribosylation factor;   

DOI  :  10.1016/0014-5793(96)00205-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An oleate dependent form of phospholipase D is present in rat brain neuronal nuclei and both the hydrolytic and transphosphatidylation activities measured. Several acidic phospholipids were found to inhibit this activity in a dose dependent manner. The IC₅₀ values varied from 3.5 μM for PIP₂ to 200 μM for phosphatidic acid. The hydrolysis of PIP₂ by phospholipase C would be expected to result in the disinhibition of the oleate dependent phospholipase D activity.

【 授权许可】

Unknown