FEBS Letters | |
Golgi apparatus mammary gland casein kinase: monitoring by a specific peptide substrate and definition of specificity determinants | |
Lasa-Benito, Marina1  Pinna, Lorenzo A.1  Marin, Oriano1  Meggio, Flavio1  | |
[1] Dipartimento di Chimica Biologica, Centro per lo Studio della Fisiologia Mitocondriale del CRIBI, Università di Padova, Via Trieste 75, 35121 Padova, Italy | |
关键词: Casein kinase; Protein kinase CK1; Protein kinase CK2; Mammary gland; Golgi apparatus; | |
DOI : 10.1016/0014-5793(96)00136-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The casein kinase from the Golgi apparatus of lactating mammary gland (GEF-CK) is distinct from ubiquitous ‘casein kinases’ termed protein kinases CK1 and CK2 and appears to define a family of secretory pathways protein kinases that phosphorylate seryl residues followed by an acidic residue at position +2. In this report we show that a new synthetic peptide substrate derived from β-casein (β[28–40]) is suitable for the fast, efficient and selective monitoring of GEF-CK, being unaffected by CK1 and CK2, and we define the consensus sequence of this protein kinase as being Ser-Xaa-Glu/SerP, distinct from that of CK2 (Ser/Thr-X-X-Glu/Asp/SerP/TyrP). In particular, the failure to recognize Asp as crucial specificity determinant prevents the phosphorylation of the specific CK2 peptide substrate RRRADDSDDDD by GEF-CK. Thus, peptide substrates are now available for the fast and specific monitoring of all the three classes of ‘casein kinases’, CK1, CK2 and GEF-CK.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020302458ZK.pdf | 604KB | download |