Generation of VV‐hemorphin‐7 from globin by peritoneal macrophages

FEBS Letters

Garreau, Isabelle¹  Sannier, Frederic¹  Zhao, Qiuyu¹  Dagouassat, Nathalie¹  Pio, Jean Marie¹ 

[1] Laboratorie de Génie Protétique et Cellulaire, Pôle Sciences et Technologies, Avenue Marillac, 17042 La Rochelle Cedex 1, France

关键词: Hemorphin;    Hemoglobin;    Macrophages;    Lysosomal enzymes;    Cathepsin D;    High-performance liquid chromatography;   

DOI  :  10.1016/0014-5793(96)00144-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Bovine globin has been incubated with mice peritoneal macrophages in order to study its hydrolysis by lysosomal enzymes, among which chiefly cathepsin D. Analysis of resulting peptides, by reversed-phase high-performance liquid chromatography (RP-HPLC), shown the release of a bioactive peptide, VV-hemorphin-7. When a carboxyl proteinase inhibitor such as pepstatin A was added, no hemorphin was generated. Our results clearly demonstrated that VV-hemorphin-7 generation was principally due to cathepsin D. This study allowed us to hypothetize a possible pathway for in vivo hemorphins appearance from globin catabolism by macrophages.

【 授权许可】

Unknown