Expression and functional characterization of a melatonin‐sensitive receptor in Xenopus oocytes

FEBS Letters

Fauteck, Jan-Dirk¹  Muβhoff, Ulrich²  Wittkowski, Werner¹  Madeja, Michael²  Fischer, Bernhard²  Speckmann, Erwin-Josef² 

[1] Institut für Anatomie, Vesaliusweg 2-4, 48149 Münster, Germany;Institut für Physiologie, Robert-Koch-Str. 27a, 48149 Münster, Germany

关键词: Melatonin;    Xenopus oocyte;    Ca2+-activated chloride current;    G protein;    IP3;    Xenopus laevis;    MEL;    melatonin;    G protein;    guanyl nucleotide binding protein;    cAMP;    cyclic adenosine 5′-monophosphate;    PTX;    pertussis toxin;    IP3;    inositol 1;    4;    5-triphosphate;    DMSO;    dimethyl sulfoxide;    9-AC;    9-anthracenecarboxylic acid;    Tris;    Tris(hydroxymethyl)aminomethane;    BSA;    bovine serum albumine;    HEPES;    N-(2-hydroxyethyl)-piperazine-N′-2-ethane sulfonic acid;   

DOI  :  10.1016/0014-5793(96)00090-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Melatonin (MEL) plays a central role in the regulation of seasonal cycles and in the control of circadian rhythms in mammals. Functional MEL-sensitive receptors were expressed in Xenopus laevis oocytes following injection of poly (A)⁺ RNA from rat brain. Administration of 0.1–100 μmol/l MEL to voltage-clamped oocytes (holding potential: −70 mV) elicited oscillatory inward currents (reversal potential: −24 mV) which could be blocked by 9-anthracenecarboxylic acid and caffeine. After preincubation with pertussis toxin (PTX) the MEL response disappeared. The expressed MEL-sensitive receptor probably activates Ca²⁺-dependent chloride currents via a PTX-sensitive G protein and the phosphoinositol pathway.

【 授权许可】

Unknown