UV‐B‐ and oxidative stress‐induced increase in nicotinamide and trigonelline and inhibition of defensive metabolism induction by poly(ADP‐ribose)polymerase inhibitor in plant tissue

FEBS Letters

Ohlsson, Anna B.²  Kalbin, Georgi¹  Rydström, Jan¹  Strid, Åke¹  Berglund, Torkel² 

[1] Department of Biochemistry and Biophysics, Göteborg University and Chalmers University of Technology, S-413 90 Gothenburg, Sweden;Department of Biochemistry and Biotechnology, Royal Institute of Technology, S-100 44 Stockholm, Sweden

关键词: Nicotinamide;    Oxidative stress;    Poly(ADP-ribose)polymerase;    Trigonelline;    UV-B;    3-AB;    3-aminobenzamide;    3-MB;    3-methoxybenzamide;    AAPH;    2;    2′-azobis(2-amidinopropane)dihydrochloride;    CHS;    chalcone synthase;    GSSG;    oxidized glutathione;    GSH;    reduced glutathione;    GSHtot;    total glutathione;    NIC;    nicotinamide;    PADPRP;    poly(ADP-ribose)polymerase;    PAL;    phenylalanine ammonia-lyase;    ROS;    reactive oxygen species;    TRIG;    trigonelline;    VOSO;   

DOI  :  10.1016/0014-5793(96)00027-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nicotinamide and trigonelline contents increased in Catharanthus roseus tissue culture after exposure to 2,2′azobis(2-amidinopropane) dihydrochloride (AAPH) or vanadylsulfate and in Pisum sativum leaves after exposure to UV-B radiation. Vanadylsulfate increased phenylalanine ammonia-lyase (PAL) activity and the content of reduced and oxidized glutathione in C. roseus tissue culture. The increases in PAL activity caused by 2 mM AAPH or 0.2 mM vanadylsulfate were prevented by 0.1 mM 3-aminobenzamide (3-AB), an inhibitor of poly(ADP-ribose)polymerase. Present results support the hypothesis [Berglund T., FEBS Lett. (1994) 351, 145–149] that nicotinamide and/or its metabolites may function as signal transmittors in the response to oxidative stress in plants and that poly(ADP-ribose)polymerase has a function in the induction of defensive metabolism.

【 授权许可】

Unknown