4‐Picoline‐2,2′:6′,2″‐terpyridine‐platinum(II) — A potent intercalator of DNA

FEBS Letters

Lowe, Gordon³  McCoubrey, Angela³  Cook, Peter R.¹  Latham, Harriet C.²  Rodger, Alison² 

[1] Sir William Dunn School of Pathology, South Parks Road, Oxford, OX1 3RE, UK;Physical Chemistry Laboratory, South Parks Road, Oxford, OX1 3QZ, UK;Dyson Perrins Laboratory, Oxford University, South Parks Road, Oxford, OX1 3QY, UK

关键词: DNA ligation;    Fluorescence spectroscopy;    Circular dichroism;   

DOI  :  10.1016/0014-5793(95)01537-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

4-Picoline-2,2′:6′,2″-terpyridine-platinum(II) is shown in a ligation assay to unwind and so intercalate into DNA. Circular dichroism is used to determine an equilibrium binding constant of approximately 2 × 10⁷ M⁻¹ for the most stable binding mode of 4-picoline-2,2′:6′,2″-terpyridine-platinum(II) to poly[d(A-T)₂] with a site size of about 4 base pairs, and about 1 × 10⁶ M⁻¹ for a second binding mode with a site size of about 2 base pairs. Fluorescence spectroscopy provides further evidence for the strong equilibrium binding constant of 4-picoline-2,2′:6′,2″-terpyridine-platinum(II) in that it displaces ethidium bromide bound to DNA. The double positive charge on 4-picoline-2,2′:6′,2″-terpyridine-platinum(II), together with the intercalative binding mode is probably responsible for the large binding constant.

【 授权许可】

Unknown