Circular dichroism and fluorescence of polyethylene glycol‐subtilisin in organic solvents

FEBS Letters

Carrea, G.¹  Riva, S.¹  Pasta, P.¹ 

[1] Istituto di Chimica degli Ormoni, CNR, Via Mario Bianco 9, 20131 Milan, Italy

关键词: Subtilisin;    Circular dichroism;    Fluorescence spectroscopy;    Organic solvent;    Polyethylene glycol;   

DOI  :  10.1016/0014-5793(88)80049-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Subtilisin Carlsberg has been made soluble in organic solvents such as dioxane and acetonitrile by covalent linking to polyethylene glycol. Far-ultraviolet circular dichroism and intrinsic protein fluorescence have shown that subtilisin dissolved in dioxane, in which the enzyme is active and highly stable, maintains the native secondary structure as well as the native microenvironment for tyrosyl residues. In acetonitrile subtilisin undergoes conformational changes that cause enzyme inactivation and precipitation.

【 授权许可】

Unknown