Characterisation of human cdc2 lysine 33 mutations expressed in the fission yeast Schizosaccharomyces pombe

FEBS Letters

Samama, Jean-Pierre¹  Leroy, Dorothée²  Birck, Catherine¹  Ducommun, Bernard²  Brambilla, Paolo³ 

[1] Structural biology group, Université Paul Sabatier, 205 route de Narbonne, 31077 Toulouse, France;Laboratoire de Pharmacologie et de Toxicologie Fondamentales du CNRS, Cell cycle group, Université Paul Sabatier, 205 route de Narbonne, 31077 Toulouse, France;USSL 63, Ospedale di Desio, 20033 Desio, Italy

关键词: Cyclin-dependent kinase;    cdc2;    Cyclin;    Schizosaccharomyces pombe;    Fission yeast;   

DOI  :  10.1016/0014-5793(95)01514-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The mammalian p34^cdc2 protein kinase, a universal cell cycle regulator, complements cdc2/CDC28 temperature-sensitive mutations in yeasts. We report the biochemical characterisation of two substitutions of human cdc2 at lysine 33, a residue involved in nucleotide binding, that differently alter the fission yeast cell cycle. K33A-hscdc2 and K33R-hscdc2 mutants are both catalytically inactive, but overexpression of K33R-cdc2 is lethal while K33A-cdc2 is not. We show that human K33R-cdc2 acts as a dominant negative allele that associates yeast cdc13/cyclinB and therefore renders endogeneous Schizosaccharomyces pombe cdc2 unactivatable. These results are discussed on the light of the molecular modeling of the mutants in the cdc2 model structure.

【 授权许可】

Unknown