FEBS Letters | |
Characterisation of human cdc2 lysine 33 mutations expressed in the fission yeast Schizosaccharomyces pombe | |
Samama, Jean-Pierre1  Leroy, Dorothée2  Birck, Catherine1  Ducommun, Bernard2  Brambilla, Paolo3  | |
[1] Structural biology group, Université Paul Sabatier, 205 route de Narbonne, 31077 Toulouse, France;Laboratoire de Pharmacologie et de Toxicologie Fondamentales du CNRS, Cell cycle group, Université Paul Sabatier, 205 route de Narbonne, 31077 Toulouse, France;USSL 63, Ospedale di Desio, 20033 Desio, Italy | |
关键词: Cyclin-dependent kinase; cdc2; Cyclin; Schizosaccharomyces pombe; Fission yeast; | |
DOI : 10.1016/0014-5793(95)01514-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The mammalian p34cdc2 protein kinase, a universal cell cycle regulator, complements cdc2/CDC28 temperature-sensitive mutations in yeasts. We report the biochemical characterisation of two substitutions of human cdc2 at lysine 33, a residue involved in nucleotide binding, that differently alter the fission yeast cell cycle. K33A-hscdc2 and K33R-hscdc2 mutants are both catalytically inactive, but overexpression of K33R-cdc2 is lethal while K33A-cdc2 is not. We show that human K33R-cdc2 acts as a dominant negative allele that associates yeast cdc13/cyclinB and therefore renders endogeneous Schizosaccharomyces pombe cdc2 unactivatable. These results are discussed on the light of the molecular modeling of the mutants in the cdc2 model structure.
【 授权许可】
Unknown
【 预 览 】
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RO201912020302267ZK.pdf | 499KB | download |