Human ClpP protease: cDNA sequence, tissue‐specific expression and chromosomal assignment of the gene

FEBS Letters

Andresen, Brage S.¹  Kruse, Torben A.²  Gregersen, Niels¹  Knudsen, Inga¹  Bross, Peter¹ 

[1] Center for Medical Molecular Biology, Aarhus University Hospital and Faculty of Health Sciences, Skejby Sygehus, Brendstrupgårdsvej, 8200 Aarhus N, Denmark;Institute for Human Genetics, Aarhus University, 8000 Aarhus C, Denmark

关键词: ATP-dependent protease;    Protein degradation;    ClpP;    cDNA sequence;    Tissue-specific expression;    Homo sapiens;    EST;    expressed sequence tag;    Hsp;    heat-shock protein;    RACE;    rapid amplification of cDNA ends;   

DOI  :  10.1016/0014-5793(95)01353-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We identified three overlapping human expressed sequence tags with significant homology to the E. coli ClpP amino sequence by screening the EMBL nucleotide database. With this sequence information we applied 5′ and 3′-rapid amplification of cDNA ends (RACE) to amplify and sequence human clpP cDNA in two overlapping fragments. The open reading frame encodes a 277 amino acid long precursor polypeptide. Two ClpP specific motifs surrounding the active site residues are present and extensive homology to ClpP's from other organisms was observed. Northern blotting showed high relative expression levels of clpP mRNA in skeletal muscle, intermediate levels in heart, liver and pancreas, and low levels in brain, placenta, lung and kidney. By analysis of human/rodent cell hybrids the human clpP gene was assigned to chromosome 19.

【 授权许可】

Unknown