Yeast mitochondrial ATP‐dependent protease: purification and comparison with the homologous rat enzyme and the bacterial ATP‐dependent protease La

FEBS Letters

Kužela, Štefan¹  SurovkovÁ, Eva¹  DurčovÁ, Gabriela¹  Kutejová, Eva¹ 

[1] Institute of Molecular Biology, Slovak Academy of Sciences, 842 51 Bratislava, Slovak Republic

关键词: Yeast mitochondria;    ATP-dependent protease;    Intramitochondrial proteolysis;    Protease La;    Saccharomyces cerevisiae;    AMPPCP;    β;    γ-methylene adenosine 5-triphosphate;    EDTA;    ethylenediaminetetraacetic acid;    KPi;    potassium phosphate;    NEM;    N-ethylmaleimide;    SDS-PAGE;    polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate;    TLCK;    Nα-p-tosyl-l-lysine chloromethyl ketone;    TPCK;    N-tosyl-l-phenylalanine chloromethyl ketone;   

DOI  :  10.1016/0014-5793(93)80190-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Homogenous ATP-dependent protease has been isolated for the first time from mitochondria of the yeast Saccharomyces cerevisiae. The enzyme molecule consists of six 120 kDa subunits. It is a serine protease with an absolute ATP requirement for its activity. Basic enzymatic characteristics of the yeast protease are similar to those of the corresponding rat mitochondrial enzyme and of the E. coli protease La. The yeast enzyme immunochemically cross-reacts with the bacterial protease La.

【 授权许可】

Unknown