Isolation of CF0CF1 from Chlamydomonas reinhardtii cw15 and the N‐terminal amino acid sequences of the CF0CF1 subunits

FEBS Letters

Strotmann, Heinrich²  Fiedler, Heike R.²  Shavit, Noun³  Leu, Stefan³  Schmid, Roland¹ 

[1] Fachbereich BiologielChemie-Mikrobiologie-, Universität Osnabrück, Osnabrück, Germany;Institut für Biochemie der Pflanzen, Heinrich Heine Univesität Düsseldorf, Universitätsstr. 1, D-40225 Düsseldorf, Germany;Center of Bioenergetics, Life Science Department, Ben Gurion University, Beer Sheva, Israel

关键词: Chlamydomonas;    CF0CF1;    Amino acid sequence;   

DOI  :  10.1016/0014-5793(95)01332-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

CF₀CF₁ was isolated from chloroplasts of the cell wall-deficient Chlamydomonas reinhardtii strain cw15. The subunit pattern was analyzed by SDS-gel electrophoresis and the N-terminal amino acid sequences of all nine subunits were determined by microsequencing. The amino acid sequences of subunits α, β, γ and ϵ match with those derived from the corresponding Chlamydomonas DNA sequences. In variance with the previously assumed N-terminus of β; however, it was found that the first 11 amino acids are lacking. The subunits δ, I, II, III and IV were identified by comparison with known sequences of homologous polypeptides of higher plant chloroplasts and cyanobacteria, respectively.

【 授权许可】

Unknown