期刊论文详细信息
FEBS Letters
Protease inhibitors block apoptosis at intermediate stages: a compared analysis of DNA fragmentation and apoptotic nuclear morphology
Maresca, V.2  Coppola, S.2  Ghibelli, L.2  Gualandi, G.1 
[1] DABAC, Università della Tuscia, 01100 Viterbo, Italy;Dipartimento di Biologia, Università di Roma Tor Vergata, via della Ricerca Scientifica, 00123 Roma, Italy
关键词: Apoptosis;    Proteases inhibitor;    DNA digestion;    Nuclear morphology;    Etoposide;    Mb;    megabases;    kb;    kilobases;    HMW;    high molecular weight;    FCS;    foetal calf serum;    PMC;    puromycin;    TBT;    tributyltin;    VP16;    etoposide;    TI;    soy-bean trypsin inhibitor;    PMSF;    phenyl-methylsulphonyl-fluoride;    IAA;    iodoacetamide;    NEM;    N-ethyl-maleimide;    TLCK;    chloromethyl ketone;    TPCK;    chloromethyl ketone;   
DOI  :  10.1016/0014-5793(95)01284-2
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
PDF
【 摘 要 】

The possible correlation between DNA digestion and changes in nuclear morphology in apoptosis was studied by blocking the apoptotic process at intermediate stages. The apoptogenic action of three drugs: etoposide, puromycin, tributyltin, was contrasted with protease inhibitors with different specificity on U937 cells. The inhibitors interfered with the development of the apoptotic features without shifting cell death to necrosis: treated cells showed abnormal morphologies, which could be recognized as intermediate stages of apoptosis; accordingly, DNA analysis showed an inhibitor-dependent block of the apoptotic DNA digestion. The comparison between size of DNA fragments and nuclear morphology suggested the following correlations: loss of normal nuclear shape with the appearence of a ≥ 2 Mb DNA band; ongoing chromatin condensation with the progressive DNA digestion up to 50 kb; nuclear fragmentation with DNA laddering. Protease inhibitors in etoposide-treated cells did not allow the formation of 700-300 kb fragments, suggesting that they possibly derive from a cell-mediated effect.

【 授权许可】

Unknown   

【 预 览 】
附件列表
Files Size Format View
RO201912020302015ZK.pdf 556KB PDF download
  文献评价指标  
  下载次数:12次 浏览次数:58次