Unusual long target duplication by insertion of intracisternal A‐particle element in radiation‐induced acute myeloid leukemia cells in mouse

FEBS Letters

Ishihara, Hiroshi¹  Tanaka, Izumi¹ 

[1] Bioregulation Research Group, National Institute of Radiological Sciences, Anagawa 4-9-1, Inage-ku, Chiba 263, Japan

关键词: Retrotransposon;    Intracisternal A-particle;    Myeloid leukemia;    Target duplication;    Interleukin-3 gene;    Genomic DNA cloning;   

DOI  :  10.1016/0014-5793(95)01262-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Retrotransmission into the IL-3/GM-CSF gene locus by the retrotransposon intracisternal A-particle (IAP) had been observed in distinct tumor cell lines. We analyzed the locus in genomes from 7 different myeloid leukemia cell strains which were originally generated by whole-body X-irradiation of the inbred C3H/He mice at a dose of 3 Gy and maintained by in vivo passage. In one leukemia cell strain out of 7 such cases, RFLP of an allele of the interleukin-3 gene was found. Sequence analysis after cloning from the genomic library showed that a type IΔ2 IAP element was inserted in the region upstream of the IL-3 gene in the head-to-head orientation. This suggests that the locus in myeloid cells is sensitive for integration of IAP elements. Additionally, an unusual long target duplication of 82 bp, 14-fold larger than normal one, was found at the junction of the element. This suggests the possibility of a radiation-induced integration mechanism which is distinct from normal retrotransmission.

【 授权许可】

Unknown