Nucleotide induced conformation determines posttranslational isoprenylation of the ras related rab6 protein in insect cells

FEBS Letters

Schiedel, Anke C.¹  Barnekow, Angelika¹  Mayer, Thomas¹ 

[1] Department for Experimental Tumorbiology, Muenster University, Badestr. 9, 48149 Muenster, Germany

关键词: GTP;    Ras protein;    Intracellular transport;    Rab proteins;    GTPase;    NSF;    N-ethylmaleimide-sensitive factor;    SNAP;    soluble NSF attachment protein;    SNARE;    SNAP receptor;    GAP;    GTPase activating protein;    GDI;    guanosine nucleotide dissociation inhibitor;    GDS;    guanosine nucleotide dissociation stimulating protein;    GTPγS;    guanosine 5′-O-thiophosphate;    REP;    rab escort protein;   

DOI  :  10.1016/0014-5793(95)01258-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Small GTP binding proteins of the rab/YPT family are essential regulators of vectorial transport in the eukaryotic cell. Members of the rab/YPT1 family are found on the cytoplasmic surface of distinct intracellular membrane compartments. Membrane attachment is facilitated by a C-terminal geranylgeranyl moiety. In this report we investigated posttranslational modification and membrane binding of the rab6 protein, a member of the rab/YPT family located on the Golgi apparatus. A set of point mutations, which simulate the GDP or GTP bound conformation, was introduced into the rab6 cDNA. The mutated cDNAs were expressed in insect cells and the ability of the protein products to undergo geranylgeranyl modification and membrane association was assessed by Triton X-114 partition and cell fractionation. We report here that the modification of rab6 in insect cells depends on protein conformation. Only the GDP bound form, but not the GTP bound form is isoprenylated and subsequently membrane bound.

【 授权许可】

Unknown