Transcriptional regulation of apolipoprotein A‐I expression in Hep G2 cells by phorbol ester

FEBS Letters

Lambert, Gilles¹  Bereziat, Gilbert¹  Mangeney-Andreani, Marise¹  Girlich, Delphine¹  Janvier, Brigitte¹  Vandenbrouck, Yves¹ 

[1] CNRS URA 1283, CHU Saint antoine, 27 rue Chaligny, 75012 Paris, France

关键词: Apolipoprotein A-I;    Phorbol ester;    Gene expression;    Hep G2 cell;    apo;    apolipoprotein;    CAT;    chloramphenicol acetyl transferase;    FCS;    fetal calf serum;    PBS;    phosphate-buffered saline;    SPAP;    secreted placental phosphatase alcaline;    TPA;    12-O-tetradecanoylphorbol 13-acetate;   

DOI  :  10.1016/0014-5793(95)01252-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The regulation of apolipoprotein A-I (apo A-I) gene expression by 12-O-tetradecanoylphorbol 13-acetate (TPA) was investigated in the human hepatoma cell line Hep G2. TPA treatment decreased apo A-I mRNA levels in a time-dependent manner, by up to 50% versus control cells within 24 h. Nuclear run-on transcription assays demonstrated a transcriptional effect of TPA. Using transfection analysis with a plasmid construct containing the −1378/+11 apo A-I promoter fused to the secreted placental alkaline phosphatase (SPAP) reporter gene, we showed that the SPAP activity was decreased to 50% when Hep G2 cells were incubated in the presence of TPA. The inhibitory effect of TPA was still maintained when fragment −253 to −4 of apo A-I promoter was linked to the CAT reporter gene. These data indicate that transcriptional modulation of apolipoprotein A-I gene expression following phorbol ester treatment is transduced by gene elements located between −253 and −4 of the apo A-I promoter.

【 授权许可】

Unknown