High hydrostatic pressure extract of garlic increases the HDL cholesterol level via up-regulation of apolipoprotein A-I gene expression in rats fed a high-fat diet

Lipids in Health and Disease

Research

In-Hwan Kim¹  Yangha Kim²  Hyunjin Joo²  Seohyun Lee²  Chong-Tai Kim³ 

[1] Department of Food and Nutrition, College of Health Science, Korea University, Seoul, Republic of Korea;Department of Nutritional Science and Food Management, Ewha Womans University, Seoul, Republic of Korea;Food Bio-Nano Research Group, Korea Food Research Institute, Seongnam, Gyeonggi, Republic of Korea;

关键词: Garlic;    High hydrostatic pressure processing;    HDL-cholesterol;    Gene expression;    Apolipoprotein A-I;    ATP-binding cassette transporter A1;    Lecithin:cholesterol acyltransferase;   

DOI  :  10.1186/1476-511X-11-77

 received in 2012-02-29, accepted in 2012-06-09,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundCardiovascular disease (CVD) is the number one cause of mortality worldwide and a low high-density lipoprotein cholesterol (HDL-C) level is an important marker of CVD risk. Garlic (Allium sativum) has been widely used in the clinic for treatment of CVD and regulation of lipid metabolism. This study investigated the effects of a high hydrostatic pressure extract of garlic (HEG) on HDL-C level and regulation of hepatic apolipoprotein A-I (apoA-I) gene expression.MethodsMale Sprague–Dawley rats were divided into two groups and maintained on a high-fat control diet (CON) or high-fat control diet supplemented with high hydrostatic pressure extract of garlic (HEG) for 5 weeks. Changes in the expression of genes related to HDL-C metabolism were analyzed in liver, together with biometric and blood parameters.ResultsIn the HEG group, the plasma triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) levels were significantly decreased in comparison with the CON group (P < 0.05). Dietary HEG also lowered the hepatic TG and total cholesterol (TC) levels compared to the CON group. While the plasma HDL-C level and mRNA level of hepatic apoA-I, which is one of primarily proteins of HDL-C particle, were significantly increased in the HEG group compared to the CON group (P < 0.05). The gene expression of ATP-binding cassette transporter A1 (ABCA1) and lecithin:cholesterol acyltransferase (LCAT), importantly involved in the biogenesis in HDL, were also up-regulated by dietary HEG.ConclusionsThese results suggest that HEG ameliorates plasma lipid profiles and attenuates hepatic lipid accumulation in the high-fat fed rats. Our findings provides that the effects of HEG on the increase of the plasma HDL-C level was at least partially mediated by up-regulation of hepatic genes expression such as apoA-I, ABCA1, and LCAT in rats fed a high-fat diet.

【 授权许可】

Unknown   
© Lee et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.