Occurrence and postmortem generation of anandamide and other long‐chain N‐acylethanolamines in mammalian brain

FEBS Letters

Schmid, Patricia C.¹  Dettmer, Timothy M.¹  Krebsbach, Randy J.¹  Maasson, Jeffrey L.¹  Perry, Stephen R.¹  Schmid, Harald H.O.¹ 

[1] Hormel Institute, University of Minnesota, 801 16th Avenue NE, Austin, MN 55912, USA

关键词: N-Acylethanolamine;    Anandamide;    Arachidonic acid;    Pig brain;    Sheep brain;    Cow brain;    NAE;    N-acylethanolamine;    TLC;    thin-layer chromatography;    GC-MS;    gas chromatography-mass spectrometry;    MSD;    mass selective detector;    t-BDMS;    tert-butyldimethylsilyl;   

DOI  :  10.1016/0014-5793(95)01194-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Long-chain N-acylethanolamines (NAEs) were assayed in pig, sheep and cow brain by gas chromatography/mass spectrometry of their tert.-butyldimethylsilyl derivatives in the presence of deuterium-labeled internal standards. Immediately after death, total NAEs ranged from about 2.7 μg/g wet weight (sheep, cow) to 6.5 μg/g wet weight (pig) and consisted almost exclusively (99%) of saturated and monounsaturated species. Anandamide (20:4n-6 NAE) comprised about 1% of total NAE in pig and cow brain, but was absent in freshly extracted sheep brain. When pig brain was analysed after 0.5, 1, 3, 4 and 23 h at ambient temperature, NAE levels were found to increase substantially over the entire time period with 20:4n-6 NAE formation exhibiting a time lag compared to that of saturated and monounsaturated NAEs.

【 授权许可】

Unknown